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Rabbit Anti-Oct4/Cy5.5 Conjugated antibody (bs-0830R-Cy5.5)
訂購(gòu)熱線:400-901-9800
訂購(gòu)郵箱:sales@xucheq.com
訂購(gòu)QQ:  400-901-9800
技術(shù)支持:techsupport@xucheq.com
說(shuō) 明 書(shū): 100ul  
100ul/2980.00元
大包裝/詢(xún)價(jià)
產(chǎn)品編號(hào) bs-0830R-Cy5.5
英文名稱(chēng)1 Rabbit Anti-Oct4/Cy5.5 Conjugated antibody
中文名稱(chēng) Cy5.5標(biāo)記的胚胎干細(xì)胞關(guān)鍵蛋白抗體
別    名 MGC22487; Oct 3; Oct 4; Oct-3; Oct-4; OCT3; Oct4; Octamer binding protein 3; Octamer-binding protein 3; Octamer binding protein 4; Octamer binding transcription factor 3; Octamer-binding transcription factor 3; Octamer-4; POU domain transcription factor OCT4; OTF3; OTF4; OTF 3; OTF 4; OTF-3; POU class 5 homeobox 1; POU domain, class 5, transcription factor 1; POU domain class 5 transcription factor 1; PO5F1_HUMAN; POU type homeodomain containing DNA binding protein ; POU5F1.  
規(guī)格價(jià)格 100ul/2980元 購(gòu)買(mǎi)        大包裝/詢(xún)價(jià)
說(shuō) 明 書(shū) 100ul  
研究領(lǐng)域 細(xì)胞生物  染色質(zhì)和核信號(hào)  信號(hào)轉(zhuǎn)導(dǎo)  干細(xì)胞  細(xì)胞周期蛋白  轉(zhuǎn)錄調(diào)節(jié)因子  表觀遺傳學(xué)  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, )
產(chǎn)品應(yīng)用 Flow-Cyt=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 39kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Octamer4
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.

Function:
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

Subunit:
Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2.

Subcellular Location:
Cytoplasm.Nucleus.Note=Expressed in a diffuse and slightly punctuate pattern.

Tissue Specificity:
Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

Post-translational modifications:
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity).
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation (By similarity).
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.

Similarity:
Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.

Database links:

Entrez Gene: 282316 Cow

Entrez Gene: 5460 Human

Entrez Gene: 18999 Mouse

Entrez Gene: 100127461 Pig

Entrez Gene: 294562 Rat

Omim: 164177 Human

SwissProt: O97552 Cow

SwissProt: Q01860 Human

SwissProt: P20263 Mouse

SwissProt: Q9TSV5 Pig

Unigene: 249184 Human

Unigene: 632482 Human

Unigene: 646545 Human

Unigene: 17031 Mouse

Unigene: 161748 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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