| 產(chǎn)品編號 | bs-8536R-Bio |
| 英文名稱1 | Rabbit Anti-Nucleolin/C23/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的核仁蛋白C23抗體 |
| 別 名 | C23; NCL; Nucl; NUCL_HUMAN; Nucleolin; Protein C23. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 染色質(zhì)和核信號 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 76kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nucleolin |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Nucleolin is the major nucleolar protein of growing eukaryotic cells. C23 (nucleolin, NCL) is a eukaryotic nucleolar phosphoprotein that influences synthesis and maturation of ribosomes. C23 localizes to dense fibrillar regions of the nucleolus. It contains four RNA binding domains that interact with pre-rRNA during synthesis. C23 can influence RNA processing, ribosomal gene transcription and nucleolar targeting of ribosomal components. It is known to associate with a variety of proteins, including the nucleolar protein B23. Phosphorylation by Cdc2 and casein kinase II causes translocation of C23 from the nucleolus to the cytoplasm. Mitotic phosphorylated forms of Bcl-2 are present in nuclear structures in prophase Hela cells together with C23 and Ki-67. Retinoic acid-induced apoptosis leads to C23 down-regulation and Bcl-2 mRNA instability. C23 binds the human telomerase reverse transcriptase subunit (TERT) through interactions with its RNA binding domain 4 and carboxyl-terminal RGG domain, and this interaction is critical for the nucleolar localization of human TERT. Function: Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Subunit: Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with APTX and NSUN2. Component of the SWAP complex that consists of NPM1, NCL/nucleolin, PARP1 and SWAP70. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts (via RRM1 and C-terminal RRM4/Arg/Gly-rich domains) with TERT; the interaction is important for nucleolar localization of TERT. Interacts with ERBB4. Interacts with GZF1; this interaction is important for nucleolar localization of GZF1. Interacts with NVL. Subcellular Location: Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Post-translational modifications: Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity). Similarity: Contains 4 RRM (RNA recognition motif) domains. Database links: Entrez Gene: 4691 Human Entrez Gene: 17975 Mouse Omim: 164035 Human SwissProt: P19338 Human SwissProt: P09405 Mouse Unigene: 79110 Human Unigene: 154378 Mouse Unigene: 474153 Mouse Unigene: 144561 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
