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Rabbit Anti-phospho-Separase (Ser1073)/Gold Conjugated antibody (bs-5304R-Gold)
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說 明 書: 100ul(10nm  15nm  35nm
100ul/2980.00元
大包裝/詢價
產品編號 bs-5304R-Gold
英文名稱1 Rabbit Anti-phospho-Separase (Ser1073)/Gold Conjugated antibody
中文名稱 膠體金標記的磷酸化外紡錘體極樣蛋白1抗體
別    名 Caspase like protein ESPL1; ESP 1; ESP1; ESP-1; ESPL 1; ESPL1; ESPL-1; Extra spindle poles like 1; Extra spindle poles like 1 protein; Separin; Similar to fission yeast cut1and gene; SSE; Separase; ESPL1_HUMAN.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul(10nm  15nm  35nm
產品類型 磷酸化抗體 
研究領域 腫瘤  免疫學  信號轉導  轉運蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, 
產品應用 IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 198kDa
性    狀 Lyophilized or Liquid
濃    度 0.4mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human ESPL1 around the phosphorylation site of Ser1073
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300.
保存條件 Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles.
產品介紹 background:
Separase is a cysteine protease that is essential for mitotic progression by separating sister chromatids. Each cell must receive one chromatid of every chromosome, during mitosis. Cohesin plays an important role in cohering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys cohesin by means of cleavage, allowing the chromatids to separate and divide with the cell. Separase activity is highly regulated. It not only cleaves cohesin at the onset of anaphase but also cleaves itself, promoting downregulation of separase after anaphase. Should a human cell become an aneuploid (one too many or too few chromatids), the embryo most likely will not survive. Should the embryo survive, it will most likely develop severe birth defects or later develop malignant cancers.

Function:
Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.

Subunit:
Interacts with PTTG1. Interacts with RAD21.

Subcellular Location:
Cytoplasmic and Nuclear

Post-translational modifications:
Autocleaves. This function, which is not essential for its protease activity, is unknown.
Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.

Similarity:
Belongs to the peptidase C50 family.

Database links:

Entrez Gene: 9700 Human

Entrez Gene: 105988 Mouse

Omim: 604143 Human

SwissProt: Q14674 Human

SwissProt: P60330 Mouse

Unigene: 153479 Human

Unigene: 288324 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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