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Rabbit Anti-ATP6V0A1/PE-Cy5 Conjugated antibody (bs-7410R-PE-Cy5)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bs-7410R-PE-Cy5
英文名稱1 Rabbit Anti-ATP6V0A1/PE-Cy5 Conjugated antibody
中文名稱 PE-Cy5標(biāo)記的氫離子轉(zhuǎn)運(yùn)ATP合成酶A1抗體
別    名 V ATPase; V ATPase A; V ATPase A1; V-ATPase A1; Vacuolar proton ATPase a1; V-type proton ATPase 95 kDa subunit a isoform 1; V-ATPase 95 kDa isoform a1; AltName: Full=Vacuolar proton pump subunit a1; Vacuolar proton translocating ATPase 95 kDa subunit a isoform 1; VPP1_HUMAN.  
規(guī)格價(jià)格 100ul/2980元 購買        大包裝/詢價(jià)
說 明 書 100ul  
研究領(lǐng)域 細(xì)胞生物  激酶和磷酸酶  通道蛋白  細(xì)胞膜受體  轉(zhuǎn)運(yùn)蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
產(chǎn)品應(yīng)用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 96kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human V-ATPase A1 (1-100aa)
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
The subunit of the vacuolar proton pump is a V-ATPase that has two different isoforms. The type I isoform contains an 18-base pair insert and is expressed in brain, whereas the truncated type II isoform is more widely expressed, including lung, kidney and spleen. The subunit of the vacuolar proton pump is located in clathrin-coated vesicles and is also found in osteoclasts. It consists of two fundamental domains, a hydrophilic amino-terminus, which has greater than 30% charged residues, and a hydrophobic carboxy terminus, which contains at least six transmembrane regions. The proton pump functions in coupling ATP hydrolysis by the cytoplasmic subunits to proton translocation by the intramembranous components of the pump. The inactivation of the osteoclast-specific vacuolar proton ATPase subunit is responsible for the lack of the enzyme in the apical membranes of osteoclast cells in osteosclerotic mutant mice, thus preventing the resorption function of these cells and leading to the osteopetrotic phenotype. The subunit, which co-localizes with the late endosomal marker Rab7 on vacuolar membranes, is essential for vacuole formation by selective swelling of late endosomes.

Function:
Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).

Subunit:
The V-ATPase is a heteromultimeric enzyme composed of at least thirteen different subunits. It has a membrane peripheral V1 sector for ATP hydrolysis and an integral V0 for proton translocation. The V1 sector comprises subunits A-H, whereas V0 includes subunits a, d, c, c', and c''.

Subcellular Location:
Cytoplasmic vesicle membrane; Multi-pass membrane protein. Melanosome. Note=Coated vesicle. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Similarity:
Belongs to the V-ATPase 116 kDa subunit family.

Database links:

Entrez Gene: 395474 Chicken

Entrez Gene: 286768 Cow

Entrez Gene: 535 Human

Entrez Gene: 11975 Mouse

Entrez Gene: 29757 Rat

Omim: 192130 Human

SwissProt: Q9I8D0 Chicken

SwissProt: Q29466 Cow

SwissProt: Q93050 Human

SwissProt: Q9Z1G4 Mouse

SwissProt: P25286 Rat

Unigene: 463074 Human

Unigene: 340818 Mouse

Unigene: 475829 Mouse

Unigene: 6015 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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