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Rabbit Anti-GEM/BF488 Conjugated antibody (bs-13331R-BF488)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產(chǎn)品編號 bs-13331R-BF488
英文名稱1 Rabbit Anti-GEM/BF488 Conjugated antibody
中文名稱 BF488標記的GTP結(jié)合絲裂原誘導T細胞蛋白抗體
別    名 GTP binding mitogen induced T cell protein; GTP binding protein expressed in mitogen stimulated T cells; GTP binding protein GEM; GTP binding protein overexpressed in skeletal muscle; Kinase inducible Ras like protein; KIR; MGC26294; RAS like protein KIR; GEM_HUMAN.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領(lǐng)域 細胞生物  信號轉(zhuǎn)導  G蛋白偶聯(lián)受體  淋巴細胞  t-淋巴細胞  G蛋白信號  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, )
產(chǎn)品應(yīng)用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 34kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GEM
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Gem belongs to the Rad/Gem/Kir (RGK) subfamily of Ras-related GTPases, which lack typical C-terminal amino acid motifs for isoprenylation. Rad and Gem bind calmodulin in a Ca2+-dependent manner via this C-terminal extension, involving residues 278–297 in human Rad. High intracellular Gem levels, which interact with intact microtubules and microfilaments, promote profound changes in cell morphology. Ectopic Gem expression is sufficient to stimulate cell flattening and neurite extension in N1E-115 and SH-SY5Y neuroblastoma cells, suggesting a role for Gem in cytoskeletal rearrangement and/or morphological differentiation of neurons. Gem was also observed in developing trigeminal nerve ganglia in 12.5 day mouse embryos, demonstrating that Gem expression is a property of normal ganglionic development. The interaction of Gem with beta-subunits regulates Ca2+ channel expression at the cell surface. The human Gem gene maps to chromosome ?8q22.1.

Function:
GEM belongs to the RAD/GEM family of GTP-binding proteins. It could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. GEM has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified.

Subunit:
Interacts with calmodulin in a Ca(2+)-dependent manner. Binds ROCK1.

Subcellular Location:
Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Tissue Specificity:
Most abundant in thymus, spleen, kidney, lung, and testis. Less abundant in heart, brain, liver and skeletal muscle.

Post-translational modifications:
Phosphorylated on tyrosine residues.

Similarity:
Belongs to the small GTPase superfamily. RGK family.

Database links:

Entrez Gene: 2669 Human

Entrez Gene: 14579 Mouse

Entrez Gene: 297902 Rat

Omim: 600164 Human

SwissProt: P55040 Human

SwissProt: P55041 Mouse

Unigene: 654463 Human

Unigene: 247486 Mouse

Unigene: 40436 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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