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Rabbit Anti-eIF3s8/PE-Cy5 Conjugated antibody (bs-14546R-PE-Cy5)
訂購熱線:400-901-9800
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產(chǎn)品編號 bs-14546R-PE-Cy5
英文名稱1 Rabbit Anti-eIF3s8/PE-Cy5 Conjugated antibody
中文名稱 PE-Cy5標記的eIF3s8蛋白抗體
別    名 Cell migration inducing protein 17; eIF 3c; EIF3 p110; eIF3-p110; eIF3c; eIF3c; EIF3C_HUMAN; EIF3CL; EIF3S8; EIF3S8 protein; Eukaryotic translation initiation factor 3 subunit 8 (110kD); Eukaryotic translation initiation factor 3 subunit 8 110kDa; Eukaryotic translation initiation factor 3 subunit 8; Eukaryotic translation initiation factor 3 subunit c.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 細胞生物  轉運蛋白  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse, Rat,  (predicted: Human, Dog, Cow, Horse, Rabbit, Sheep, )
產(chǎn)品應用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 105kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human eIF3s8
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts (1,2). Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation (1-3). Eukaryotic initiation factor-3 (eIF3) is the largest family of eIFs and consists of at least 10 unique subunits (p170, p116, p110, p66, p48, p47, p44, p40, p36, and p35) in mammals (3,4). eIF3 subunit-9 (eIF3-h, eIF3-p116, p116, eIF3-S9, PRT1) is a 873 amino acid component of the eIF3 multi-subunit complex that is involved in ribosomal 48S complex formation (3,5). Association of the eIF3 complex with the 40S ribosomal subunit stabilizes eIF2-GTP-Met-tRNAiMet complex association and mRNA binding, and promotes dissociation of 80S ribosomes into 40S and 60S subunits (6,7).

Function:
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Subunit:
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Interacts with ALKBH4, IFIT1 and IFIT2.

Subcellular Location:
Cytoplasm.

Post-translational modifications:
Phosphorylated. Phosphorylation is enhanced upon serum stimulation.

Similarity:
Belongs to the eIF-3 subunit C family.
Contains 1 PCI domain.

Database links:

Entrez Gene: 8663 Human

Entrez Gene: 56347 Mouse

Entrez Gene: 293484 Rat

Omim: 603916 Human

SwissProt: Q99613 Human

SwissProt: Q8R1B4 Mouse

SwissProt: B5DFC8 Rat

Unigene: 567374 Human

Unigene: 22776 Mouse

Unigene: 4207 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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