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Mouse Anti-phospho-MLKL (Ser358)/Gold Conjugated antibody (bsm-33331M-Gold)
訂購(gòu)熱線:400-901-9800
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說(shuō) 明 書(shū): 100ul(10nm  15nm  35nm
100ul/2980.00元
大包裝/詢(xún)價(jià)
產(chǎn)品編號(hào) bsm-33331M-Gold
英文名稱(chēng)1 Mouse Anti-phospho-MLKL (Ser358)/Gold Conjugated antibody
中文名稱(chēng) 膠體金標(biāo)記的磷酸化MLKL單克隆抗體
別    名 phospho-MLKL(S358); p-MLKL(S358); MLKL(p-S358);9130019I15Rik; FLJ34389; hMLKL; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain-like protein; MLKL_HUMAN.  
規(guī)格價(jià)格 100ul/2980元 購(gòu)買(mǎi)        大包裝/詢(xún)價(jià)
說(shuō) 明 書(shū) 100ul(10nm  15nm  35nm
研究領(lǐng)域 細(xì)胞生物  激酶和磷酸酶  
抗體來(lái)源 Mouse
克隆類(lèi)型 Monoclonal
克 隆 號(hào) 8C4
交叉反應(yīng)
產(chǎn)品應(yīng)用 IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 54kDa
性    狀 Lyophilized or Liquid
濃    度 0.4mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human phospho-MLKL (Ser358)
亞    型 IgG
純化方法 affinity purified by Protein G
儲(chǔ) 存 液 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300.
保存條件 Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles.
產(chǎn)品介紹 background:
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015].

Function:
Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.

Subunit:
Homotrimer; forms homotrimers on necroptosis induction. Interacts with RIPK3; the interaction is direct. Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3. Within this complex, may play a role in the proper targeting of RIPK1/RIPK3 to its downstream effector PGAM5.

Subcellular Location:
Cytoplasm. Cell membrane. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.

Post-translational modifications:
Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.

Similarity:
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain.

Database links:

Entrez Gene: 197259 Human

Entrez Gene: 74568 Mouse

Entrez Gene: 690743 Rat

Omim: 615153 Human

SwissProt: Q8NB16 Human

SwissProt: Q9D2Y4 Mouse

Unigene: 119878 Human

Unigene: 207971 Mouse

Unigene: 105677 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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