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Mouse Anti-Cytokeratin 8/FITC Conjugated antibody (bsm-33061M-FITC)
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說(shuō) 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bsm-33061M-FITC
英文名稱1 Mouse Anti-Cytokeratin 8/FITC Conjugated antibody
中文名稱 FITC標(biāo)記的細(xì)胞角蛋白8單克隆抗體
別    名 card2; Cardiac autoantigen 2 120kD; CK 8; CK8; CK-8; ck8; Cyk 8; cyk8; CYKER; Cytokeratin endo A; Cytokeratin-8; Cytokeratin8; DreK8; EndoA; k0; CYK8; k2c8; K2C8_HUMAN; k8; Keratin 8; Keratin type ii cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; Keratin8; KO; Krt 2.8; Krt 8; krt8; KRT-8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8.  
規(guī)格價(jià)格 100ul/2980元 購(gòu)買        大包裝/詢價(jià)
說(shuō) 明 書 100ul  
研究領(lǐng)域 腫瘤  細(xì)胞生物  信號(hào)轉(zhuǎn)導(dǎo)  
抗體來(lái)源 Mouse
克隆類型 Monoclonal
克 隆 號(hào) 10A8
交叉反應(yīng) Human, Mouse, Rat, 
產(chǎn)品應(yīng)用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 53kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human CK8
亞    型 IgG
純化方法 affinity purified by Protein G
儲(chǔ) 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012].

Function:
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Subunit:
Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1.

Subcellular Location:
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.

Tissue Specificity:
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

Post-translational modifications:
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

DISEASE:
Defects in KRT8 are a cause of cirrhosis (CIRRH) [MIM:215600].

Similarity:
Belongs to the intermediate filament family.

Database links:

Entrez Gene: 3856 Human

Entrez Gene: 16691 Mouse

Entrez Gene: 25626 Rat

Omim: 148060 Human

SwissProt: P05787 Human

SwissProt: P11679 Mouse

SwissProt: Q10758 Rat

Unigene: 533782 Human

Unigene: 708445 Human

Unigene: 358618 Mouse

Unigene: 11083 Rat 



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

結(jié)構(gòu)蛋白(Structural Proteins)
上皮細(xì)胞胞質(zhì)中的骨架蛋白除微絲(肌動(dòng)蛋白)、微管蛋白外,主要是細(xì)胞角蛋白(cytokeratins,CK)。CK8也是上皮細(xì)胞的特征性標(biāo)記物:CK8存在于某些正常腺上皮及其腫瘤,包括許多導(dǎo)管上皮和腺上皮,如結(jié)腸、胃、小腸、氣管的上皮和尿路上皮。CK8主要用于腺癌和導(dǎo)管癌的診斷,鱗狀細(xì)胞癌一般不表達(dá)CK8。
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