| 產(chǎn)品編號 | bsm-30069M-APC-HRP |
| 英文名稱1 | Mouse Anti-human CD8-APC/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的APC標記小鼠抗人CD8單克隆抗體 |
| 別 名 | CD8-b;CD 8A; CD 8B; CD8 antigen alpha polypeptide; CD8A anti; CD8a antigen an; CD8a molecule; CD8B; CD8b antigen; CD8b antigen isoform 2; CD8b molecule; CD8B protein; CD8B1; Leu2; Leu2 T lymphocyte antigen; Ly3; LYT3; MAL; MGC119115; OKT8 T Cell antigen; p32; T cell antigen Leu2; T cell surface glycoprotein CD8 alpha chain; T cell surface glycoprotein CD8 beta chain; T lymphocyte differentiation antigen T8/Leu 2; CD8A_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 免疫學 干細胞 細胞表面分子 淋巴細胞 t-淋巴細胞 b-淋巴細胞 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 交叉反應(yīng) | Human, |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 27kDa |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 儲 存 液 | Constituents: 0.01M PBS, pH 7.4 with 10 mg/mL BSA and 0.1% Gentamicin, 50% glycerol. Or Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10 mg/mL BSA and 0.1% Gentamicin. Reconstitute with sterile distilled water. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]. Subunit: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
