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Rabbit Anti-MCL1/Cy5.5 Conjugated antibody (bs-23315R-Cy5.5)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產(chǎn)品編號 bs-23315R-Cy5.5
英文名稱1 Rabbit Anti-MCL1/Cy5.5 Conjugated antibody
中文名稱 Cy5.5標(biāo)記的髓樣細(xì)胞白血病-1抗體
別    名 myeloid cell leukemia 1; myeloid cell leukemia sequence 1; MCL-1; MCL1L; MCL 1; mcl1/EAT; MGC104264; MGC1839; TM; MCL1S; EAT) Bcl 2 related protein EAT/mcl1; BCL2 related; BCL2L3; EAT; Induced myeloid leukemia cell differentiation protein Mcl 1; myeloid cell leukemia sequence 1; Myeloid cell leukemia sequence 1 BCL2 related; Myeloid cell leukemia sequence 1 isoform 1; OTTHUMP00000032794; OTTHUMP00000032795; TM; bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1-ES; mcl1/EAT.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領(lǐng)域 腫瘤  免疫學(xué)  信號轉(zhuǎn)導(dǎo)  細(xì)胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  線粒體  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat,  (predicted: Dog, Pig, Horse, Rabbit, )
產(chǎn)品應(yīng)用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 39kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MCL1
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Mcl1 is an anti-apoptotic member of Bcl2 family originally isolated from the ML1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway. Mcl1 localizes to the mitochondria, interacts with and antagonizes pro-apoptotic Bcl2 family members, and inhibits apoptosis by a number of cytotoxic stimuli. It is involved in programing of differentiation and concomitant maintenance of viability but not of proliferation. Isoform 1 inhibits apoptosis while isoform 2 promotes it. Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML1.

Function:
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

Subunit:
Interacts with BAD, BOK, BIK and BFM (By similarity). Interacts with PMAIP1. Isoform 1 interacts with BAX, BAK1, TPT1 and BCL2L11. Heterodimer of isoform 1 and isoform 2. Homodimers of isoform 1 or isoform 2 are not detected. Isoform 2 does not interact with pro-apoptotic BCL2-related proteins.

Subcellular Location:
Membrane; Single-pass membrane protein (Potential). Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Note=Cytoplasmic, associated with mitochondria.

Post-translational modifications:
Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.

Similarity:
Belongs to the Bcl-2 family.

Database links:

Entrez Gene: 4170 Human

Entrez Gene: 17210 Mouse

Entrez Gene: 60430 Rat

Omim: 159552 Human

SwissProt: Q07820 Human

SwissProt: P97287 Mouse

SwissProt: Q9Z1P3 Rat

Unigene: 632486 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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