| 產(chǎn)品編號(hào) | bs-23810R-APC |
| 英文名稱1 | Rabbit Anti-Histone H4/APC Conjugated antibody |
| 中文名稱 | APC標(biāo)記的組蛋白H4抗體 |
| 別 名 | H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN; Osteogenic growth peptide; OGP. |
| 規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買(mǎi) 大包裝/詢價(jià) |
| 說(shuō) 明 書(shū) | 100ul |
| 研究領(lǐng)域 | |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Mouse, (predicted: Human, Rat, ) |
| 產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 11kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Histone H4 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.05M PB, pH 7.5. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008]. Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Subcellular Location: Nucleus. Chromosome. Post-translational modifications: Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Sumoylated, which is associated with transcriptional repression. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Similarity: Belongs to the histone H4 family. Database links: Entrez Gene: 121504 Human Entrez Gene: 554313 Human Entrez Gene: 8294 Human Entrez Gene: 8359 Human Entrez Gene: 8360 Human Entrez Gene: 8361 Human Entrez Gene: 8362 Human Entrez Gene: 8363 Human Entrez Gene: 8364 Human Entrez Gene: 8365 Human Entrez Gene: 8366 Human Entrez Gene: 8367 Human Entrez Gene: 8368 Human Entrez Gene: 8370 Human Entrez Gene: 100041230 Mouse Entrez Gene: 100862646 Mouse Entrez Gene: 319155 Mouse Entrez Gene: 319156 Mouse Entrez Gene: 319157 Mouse Entrez Gene: 319158 Mouse Entrez Gene: 319159 Mouse Entrez Gene: 319160 Mouse Entrez Gene: 319161 Mouse Entrez Gene: 320332 Mouse Entrez Gene: 326619 Mouse Entrez Gene: 326620 Mouse Entrez Gene: 69386 Mouse Entrez Gene: 97122 Mouse GenBank: NM_003548 Human Omim: 142750 Human SwissProt: P02304 Human SwissProt: P62805 Human SwissProt: P02304 Mouse SwissProt: P62806 Mouse Unigene: 143080 Human Unigene: 247816 Human Unigene: 248172 Human Unigene: 248178 Human Unigene: 248179 Human Unigene: 278483 Human Unigene: 352191 Human Unigene: 46423 Human Unigene: 528055 Human Unigene: 533295 Human Unigene: 55468 Human Unigene: 591790 Human Unigene: 655235 Human Unigene: 662174 Human Unigene: 706635 Human Unigene: 742244 Human Unigene: 14775 Mouse Unigene: 158272 Mouse Unigene: 227295 Mouse Unigene: 228709 Mouse Unigene: 246720 Mouse Unigene: 255646 Mouse Unigene: 260530 Mouse Unigene: 261642 Mouse Unigene: 261662 Mouse Unigene: 261664 Mouse Unigene: 377875 Mouse Unigene: 442307 Mouse Unigene: 486099 Mouse Unigene: 489077 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
