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Rabbit Anti-CD4/BF350 Conjugated antibody (bs-23539R-BF350)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價(jià)
產(chǎn)品編號(hào) bs-23539R-BF350
英文名稱1 Rabbit Anti-CD4/BF350 Conjugated antibody
中文名稱 BF350標(biāo)記的CD4抗體
別    名 CD4 (L3T4); CD4 antigen (p55); CD4 Antigen ; CD4 molecule; CD4 Receptor; CD4+ Lymphocyte deficiency, included; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; p55; T Cell Antigen T4 ; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T cell OKT4 deficiency, included; T cell surface antigen T4/Leu 3 ; T cell surface antigen T4/Leu3; T Cell Surface Glycoprotein CD4; W3/25; W3/25 antigen; T-cell surface glycoprotein CD4 isoform 1 precursor; CD4_MOUSE.  
規(guī)格價(jià)格 100ul/2980元 購買        大包裝/詢價(jià)
說 明 書 100ul  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  干細(xì)胞  細(xì)胞表面分子  淋巴細(xì)胞  t-淋巴細(xì)胞  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Mouse, 
產(chǎn)品應(yīng)用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 48kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse CD4
亞    型 IgG
純化方法 affinity purified by Protein A
儲(chǔ) 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010].

Function:
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

Subunit:
Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.

Post-translational modifications:
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

Similarity:
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Database links:

Entrez Gene: 920 Human

Entrez Gene: 12504 Mouse

Omim: 186940 Human

SwissProt: P06332 Mouse

SwissProt: P01730 Human

Unigene: 631659 Human

Unigene: 2209 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

此抗體可識(shí)別55KDⅠ型單鏈穿膜糖蛋白。 CD4分子是存在于大多數(shù)輔助/誘導(dǎo)T細(xì)胞表面的59kDa的糖蛋白。正常淋巴組織中CD4的表達(dá)數(shù)量多于CD8,此抗體主要用于標(biāo)記輔助/誘導(dǎo)T細(xì)胞,與CD8單抗聯(lián)合使用對(duì)外周血淋巴細(xì)胞分型。 CD4抗原是HLA-II類分子和人類免疫缺陷病毒(HIV)-愛滋病的受體,在35-50%外周血淋巴細(xì)胞-輔助和誘導(dǎo)T細(xì)胞(Th/Ti)和70-80%人胸腺細(xì)胞上表達(dá),在人的單核細(xì)胞表面也有低密度的表達(dá)。 CD4抗原有膜結(jié)合型和可溶性兩種形式。Th/Ti可輔助Ig產(chǎn)生和T細(xì)胞毒T細(xì)胞的作用
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